Journal: PLoS ONE

Article Title: Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans

doi: 10.1371/journal.pone.0069316

Figure Lengend Snippet: (a) The panel shows the different mixtures of DNA (100 ng) and MMGP1 (1- 0.036; 2- 0.072; 3- 0.144; 4- 0.288; 5-0.576 µM; C-without peptide) run on a 1% agarose gel. (b) Quantitative assessment of unbound plasmid DNA to the peptide by gel densitometry analysis.

Article Snippet: The plasmid DNA Bluescript II SK (+) was purified using a QIAprep Spin Minprep kit (Qiagen, Germay) and used for subsequent analysis.

Techniques: Agarose Gel Electrophoresis, Plasmid Preparation

Journal: PLoS ONE

Article Title: Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans

doi: 10.1371/journal.pone.0069316

Figure Lengend Snippet: (a) Measurement of intrinsic tryptophan fluorescence of MMGP1 on addition of plasmid DNA (b) Quenching of SYTO9 fluorescence in the presence of varying concentrations of MMGP1.

Article Snippet: The plasmid DNA Bluescript II SK (+) was purified using a QIAprep Spin Minprep kit (Qiagen, Germay) and used for subsequent analysis.

Techniques: Fluorescence, Plasmid Preparation

Journal: PLoS ONE

Article Title: Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans

doi: 10.1371/journal.pone.0069316

Figure Lengend Snippet: (a) Treatment of DNA–MMGP1 complexes with proteinase K resulted in detection of plasmid DNA band on the agarose gel. C-Control SK+ plasmid DNA; 1-DNA–MMGP1 complex formed at 0.576 µM concentration of peptide; 2-DNA–MMGP1 complex treated with proteinase K (b) DNase I protection assay. The plasmid DNA (100 ng) was preincubated with the varying concentrations peptides such as, C+-no peptide; 1-0.576 µM; 2-0.288 µM; 3- 0.144 μM; 4-0.072 µM; 5-0.036 µM, respectively followed by treatment with DNase I. The DNase treated plasmid DNA was used as negative control (C-).

Article Snippet: The plasmid DNA Bluescript II SK (+) was purified using a QIAprep Spin Minprep kit (Qiagen, Germay) and used for subsequent analysis.

Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Control, Concentration Assay, Negative Control